產(chǎn)品屬性：
產(chǎn)品名稱 | Thrombin (MW 37kDa) |
規(guī)格 | 1000U、2000U |
貨號 | EY-01Y13979 |
Cas No.: 9002-04-4
別名: N/A
化學名: N/A
分子式: N/A
分子量: 37000(Average)
溶解度: N/A
儲存條件: 4°C, protect from light
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
產(chǎn)品描述：
Thrombin (MW 37kDa) is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.One unit of enzyme cleaves > 90% of 100 μg of a test GST-tagged protein when incubated in 1× PBS at 22°C for 16 h.Thrombin solution: Dissolve 500 units in 0.5 mL of PBS prechilled to 4°C. Swirl gently. Store solution in small aliquots at -80°C to preserve activity.Cleavage buffer: PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4) pH 7.3.Elution buffer: 50 mM Tris-HCl, 10 mM reduced glutathione, pH 8.0[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4.
[2]. Krenzlin H, et al. The Importance of Thrombin in Cerebral Injury and Disease. Int J Mol Sci. 2016 Jan 11;17(1).
特別提醒公司產(chǎn)品僅供科研使用
